BT-549-Luc cell line

Luciferase is the general name of a class of enzymes that catalyze the oxidation and luminescence of luciferin or fatty aldehydes in organisms. Currently, firefly luciferase is the most commonly used enzyme in scientific research. This enzyme can catalyze the oxidative decarboxylation of D2 luciferin, produce activated oxidized luciferin, and emit reddish biological fluorescence. Because luciferase is based on the mutual reaction between enzyme and substrate, it has the characteristics of strong specificity, high sensitivity and signal-to-noise ratio, as well as strong penetration ability of biological fluorescent tissue. Ubigene's BT-549-Luc cell line was constructed on the basis of wild-type BT-549 cells by lentiviral transduction, which can stably express firefly luciferase for a long time. This cell line has low passage, high activity and good condition, and can be directly used for in vivo cell injection, which can well help you with the research of transcription factor regulation mechanism, in vivo imaging, cell tracking and other experiments.